Application of the Taguchi Design for Production of Poly(β-hydroxybutyrate) by Ralstonia eutropha

Authors

  • Ebrahim Vasheghani Farahani Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University P.O. Box 14115-143, Tehran, I.R. IRAN
  • Kianoush Khosravi Darani Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University P.O. Box 14115-143, Tehran, I.R. IRAN
  • Saied Abbas Shojaosadati Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University P.O. Box 14115-143, Tehran, I.R. IRAN
Abstract:

The Taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(PHB) production by Ralstonia eutropha. The optimum condition was obtained as: fructose concentration, 15 g/L; C/N ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° C; seed age, 15 h. At optimum condition the yield of PHB production was found to be 92.36%.

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Journal title

volume 23  issue 1

pages  131- 136

publication date 2004-04-01

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